THE IDENTIFICATION OF “DIRECT-REACTING” BILIRUBIN AS BILIRUBIN GLUCURONIDE
نویسندگان
چکیده
منابع مشابه
The identification of direct-reacting bilirubin as bilirubin glucuronide.
It has long been recognized that, with p-diazobenzenesulfonic acid (van den Bergh reagent), bilirubin in serum of patients with jaundice may exhibit two types of reaction (1, 2). If coupling with the diazo reagent occurs promptly in aqueous solution, the reaction is said to be “direct;” if, however, coupling t.akes place only after prior addition of alcohol, the reaction is said to be “indirect...
متن کاملThe Identification of “direct-reacting” Bilirubin as Bilirubin Glucuronide*
It has long been recognized that, with p-diazobenzenesulfonic acid (van den Bergh reagent), bilirubin in serum of patients with jaundice may exhibit two types of reaction (1, 2). If coupling with the diazo reagent occurs promptly in aqueous solution, the reaction is said to be “direct;” if, however, coupling t.akes place only after prior addition of alcohol, the reaction is said to be “indirect...
متن کاملDetermination of bilirubin glucuronide and assay of glucuronyltransferase with bilirubin as acceptor.
1. Conjugated bilirubin is conveniently determined by coupling with the diazonium salt of ethyl anthranilate. 2. This method has been used in the development of assays for UDP-glucuronyltransferase (EC 2.4.1.17), with bilirubin as substrate, in rat liver homogenates, microsomal preparations and partly purified fractions. 3. Chromatographic analysis suggests that bilirubin monoglucuronide is the...
متن کاملDirect bilirubin higher than total bilirubin?
Both total and direct bilirubin measure the formation of azobilirubin, which is detected at 570/660 nm. The total bilirubin reaction contains surfactant and caffeine, which solubilize “indirect” bilirubin and accelerate the reaction (1). Direct bilirubin is a component of total bilirubin and therefore should always be smaller in value. The presence of a monoclonal immunoglobulin (2220mg/dL) fal...
متن کاملMeasurement of direct bilirubin by use of bilirubin oxidase.
We developed an enzymatic method for measuring direct-reacting bilirubin (DBIL) in serum. At pH 4.5, bilirubin oxidase (BOX) oxidizes mono-conjugated bilirubin, di-conjugated bilirubin, and most of the delta-bilirubin to biliverdin. The resulting decrease in absorbance at 460 nm is linearly related to the concentration of DBIL in serum. Mean DBIL values in the 51 patients' sera examined by the ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1957
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)63692-6